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KMID : 0364819910290030155
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Korean Journal of Microbiology
1991 Volume.29 No. 3 p.155 ~ p.159
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Cloning of Catechol 2,3-dioxygenase Gene from Pseudomonas putida
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Abstract
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1
Four strains of Pseudomonas putida (NAH), Pseudomonas sp. (TOL), Achromobacter xylosoxidans, and Alcaligrnes sp. were compared with their degradative capability of aromatic compounds. All of the bacterial strains were utilized catechol as a sole carbon source for growth, but significantly different in degradative properties for 5 other aromatic compounds. Catechol 2, 3-dioxygenase gene from P. putida (NAH) has been cloned and expressed in E. coli. The DNA clone designated pCNU101 contains NAH-derived 6 Kb insert and its physical map was characterized. A subclone (pCNU106) for the catechol dioxygenase gene in pCNU1OI contained 2.0 kb-DNA insert fragmented by Hpal and Clal.
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